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rip-chip platform  (Ribonomics Inc)

 
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    Ribonomics Inc rip-chip platform
    Validation of <t>RIP-Chip</t> data ( a ) RT-qPCR on RNA isolated <t>from</t> <t>hnRNP</t> A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.
    Rip Chip Platform, supplied by Ribonomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/platform+rip-chip+assay/pmc03821614-337-13-12?v=Ribonomics+Inc
    Average 90 stars, based on 1 article reviews
    rip-chip platform - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes"

    Article Title: HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms141020256

    Validation of RIP-Chip data ( a ) RT-qPCR on RNA isolated from hnRNP A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.
    Figure Legend Snippet: Validation of RIP-Chip data ( a ) RT-qPCR on RNA isolated from hnRNP A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.

    Techniques Used: Quantitative RT-PCR, Isolation, Microarray, Expressing, Agarose Gel Electrophoresis, Amplification, Positive Control



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    Validation of <t>RIP-Chip</t> data ( a ) RT-qPCR on RNA isolated <t>from</t> <t>hnRNP</t> A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.
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    Validation of <t>RIP-Chip</t> data ( a ) RT-qPCR on RNA isolated <t>from</t> <t>hnRNP</t> A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.
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    Validation of RIP-Chip data ( a ) RT-qPCR on RNA isolated from hnRNP A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.

    Journal: International Journal of Molecular Sciences

    Article Title: HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

    doi: 10.3390/ijms141020256

    Figure Lengend Snippet: Validation of RIP-Chip data ( a ) RT-qPCR on RNA isolated from hnRNP A1 IPs and total nuclear extracts was performed to calculate the enrichment of mRNA for 8 target genes in HuR WT and HuR KO cells, namely Hoxc10 , Rad23a , HnRNP A1 , Gbp2 , Ccnh , Mea1 and Tceal5 and compared to the microarray analysis; ( b ) Gapdh mRNA was used for normalization. The signal for Tceal5 mRNA transcript in HuR WT cells remained at the background level. Values represent the mean of three independent experiments performed in triplicates and error bars the standard deviations; ( c ) Fold change in expression of the selected genes in HuR KO as compared to HuR WT cells as determined by RT-qPCR on total nuclear RNA from the two cell types. Values and error bars are as described above. Genes with an expression fold change <1 correspond to down-regulated genes and are graphically shown in the opposite direction; ( d ) Agarose gel electrophoresis of the products of two RT-qPCR reactions, namely for Tceal5 and Mea1 transcripts, indicating that Tceal5 mRNA was undetected in HuR WT cells even after extension of the reaction cycles. The amplicon corresponding to Mea1 is shown as a positive control.

    Article Snippet: To this extent, we report here on the novel application of the Ribonomics (RIP-Chip) platform on hnRNP A1-RNP complexes immunoselected from the nuclear extracts of HuR-lacking and HuR-containing mouse embryonic fibroblast (MEF) cells.

    Techniques: Quantitative RT-PCR, Isolation, Microarray, Expressing, Agarose Gel Electrophoresis, Amplification, Positive Control